Crystal form of azaindole derivative and use thereof

ABSTRACT

A crystal form of an azaindole derivative and a preparation method thereof are disclosed.

THE PRESENT APPLICATION CLAIMS THE FOLLOWING PRIORITY

CN202010042713.X with filing date of 2020-01-15

FIELD OF TECHNOLOGY

The present invention relates to a crystal form of an azaindole derivative and a preparation method thereof.

BACKGROUND

Fibroblast growth factor receptors (FGFRs) are a class of biologically active substances having functions such as transducing biological signals, regulating cell growth, and participating in tissue repair. In recent years, several members of the FGFR family are found to play an important role in tumor formation and development. FGFRs are a class of receptor proteins that can specifically bind to fibroblast growth factors (FGFs). The FGFR family includes FGFR1b, FGFR1c, FGFR2b, FGFR2c, FGFR3b, FGFR3c, and FGFR4. Different subtypes of FGFRs bind to different FGFs. The binding of FGFs to FGFRs leads to the autophosphorylation of multiple tyrosine residues in the cells. The phosphorylated FGFRs activate downstream signaling pathways including MEK/MAPK, PLCy/PKC, PI3K/AKT, and STATS, etc. In tumors, such as liver cancer, bladder cancer, lung cancer, breast cancer, endometrial cancer, glioma, prostate cancer, and so on, the activating mutations of FGFRs or ligand/receptor overexpression causes persistent constitutive activation, which is closely related to the occurrence, development, and poor prognosis of tumors, and also plays an important role in tumor angiogenesis, tumor invasion and metastasis. Therefore, FGFRs are considered to be an important therapeutic target of tumors.

c-Met protein (also known as hepatocyte growth factor (HGF) receptor) is a 190-kDa transmembrane heterodimer with tyrosine kinase activity, encoded by the c-Met oncogene. c-MET is the only known receptor of HGF, and the binding of HGF to c-MET can activate the downstream signaling cascades, to phosphorylate the cytoplasmic tyrosine kinase and then cause the autophosphorylation of MET. Various cytoplasmic effector proteins, including GRB2, GAB1, PLC and SOS, are recruited and phosphorylated. Once activated, GAB1 forms a binding site for downstream proteins (PI3K, etc.). It enters the nucleus through the RAS-MAPK and PI3K-AKT signaling pathways and thus affects the gene expression and cell cycle progression. It has been shown that the HGF/c-Met signaling pathway exhibits various cellular responses, including mitogenic activity, proliferative activity, morphogenic activity, and angiogenic activity. c-Met protein is abnormal in about 5-10% of patients with tumors, including patients of liver cancer, gastric cancer, non-small cell lung cancer, bladder cancer, breast cancer, colorectal cancer, head and neck squamous cell carcinoma, hypopharyngeal cancer, ovarian cancer, and others. Inhibitors of the HGF/c-Met pathway are clinically confirmed to have significant potential in the treatment of cancers. The Patent WO 2010059771 reports a small molecule inhibitor with activities against c-Met.

FGFR and c-Met are both members of the receptor tyrosine kinase (RTK) family, and the signaling pathways PI3K-AKT-mTOR and RAS-RAF-MEK-ERK are regulated by the two together. Numerous studies have demonstrated that tumor escape occurs between the FGFR and c-Met targets.

In terms of the molecular mechanism of action, FGFR and FGFR are both members of the receptor tyrosine kinase (RTK) family, and the signaling pathways PI3K-AKT-mTOR and RAS-RAF-MEK-ERK are regulated by the two together. The FGFR and c-Met targets are synergistic and complementary, and the FGFR mutation and c-Met mutation tend to have a signal compensation effect when the other is inhibited, thus making the tumor cells resistant to a single inhibitor.

The Patent WO2010059771A1 discloses Met and RON inhibitors as described in Comparative Examples 1a and 1b. At present, no dual small molecule inhibitor with high activity against both FGFR and c-Met is found.

SUMMARY

The present invention provides a compound of Formula (II):

The present invention further provides a crystal form A of the compound of Formula (II), having an X-ray powder diffraction (XRPD) pattern with characteristic diffraction peaks at 2θ of 5.40°±0.20°, 11.99°±0.20°, and 14.77°±0.20°.

In some embodiments of the present invention, the crystal form A has an XRPD pattern with characteristic diffraction peaks at 2θ of 5.40±0.20°, 10.77±0.20°, 11.99±0.20°, 14.77±0.20°, 21.55±0.20°, 23.25±0.20°, 24.14±0.20°, and 27.69±0.20°.

In some embodiments of the present invention, the crystal form A has an XRPD pattern with characteristic diffraction peaks at 2θ of 5.402°, 8.949°, 10.766°, 11.989°, 13.186°, 14.766°, 16.090°, 16.779°, 19.721°, 21.554°, 23.251°, 23.685°, 24.138°, 25.224°, 27.690°, 28.670°, 29.287°, 31.378°, 33.941°, and 38.046°.

In some embodiments of the present invention, the crystal form A has an XRPD as shown in FIG. 1 .

In some embodiments of the present invention, the resolved data from the XRPD pattern of the crystal form A are shown in Table 1:

TABLE 1 Resolved data from the XRPD pattern of the crystal form A No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) 1 5.402 16.3462 100.0 11 23.251 3.8224 49.7 2 8.949 9.8734 15.1 12 23.685 37534 31.9 3 10.766 8.2108 62.3 13 24.138 3.6839 43.7 4 11.989 7.3758 98.4 14 25.224 3.5278 13.6 5 13.186 6.7087 16.2 15 27.690 3.2189 28.3 6 14.766 5.9943 99.6 16 28.670 3.1111 8.1 7 16.090 5.5040 17.2 17 29.287 3.0469 12.0 8 16.779 5.2794 18.0 18 31.378 2.8485 8.1 9 19.721 4.4980 148 19 33.941 2.6391 7.5 10 21.554 4.1195 55.7 20 38.046 2.3632 6.9

In some embodiments of the present invention, the crystal form A has a differential scanning calorimetry (DSC) profile with an endothermic peak starting at 220.0±3.0° C.

In some embodiments of the present invention, the crystal form A has a DSC profile as shown in FIG. 2 .

In some embodiments of the present invention, the crystal form A has a thermogravimetric analysis (TGA) curve showing a weight loss of 1.04% at 150.0° C.±3.0° C.

In some embodiments of the present invention, the crystal form A has a TGA curve as shown in FIG. 3 .

The present invention further provides a crystal form B of the compound of Formula (II), having an XRPD pattern with characteristic diffraction peaks at 2θ of 14.89°±0.20°, 21.00°±0.20°, and 26.74°±0.20°.

In some embodiments of the present invention, the crystal form B has an XRPD pattern with characteristic diffraction peaks at 2θ of 12.07±0.20°, 14.89±0.20°, 21.00±0.20°, 21.70±0.20°, 24.34±0.20°, 26.74±0.20°, 27.59±0.20°, and 28.10±0.20°.

In some embodiments of the present invention, the crystal form B has an XRPD pattern with characteristic diffraction peaks at 2θ of 5.462°, 10.806°, 11.042°, 12.067°, 12.700°, 13.328°, 14.890°, 16.010°, 17.194°, 18.300°, 18.887°, 19.933°, 21.000°, 21.695°, 24.336°, 24.632°, 26.742°, 27.589°, 28.104°, 28.931°, 29.639°, 34.213°, and 35.560°.

In some embodiments of the present invention, the crystal form B has an XRPD pattern as shown in FIG. 4 .

In some embodiments of the present invention, the resolved data from the XRPD pattern of the crystal form B are shown in Table 2:

TABLE 2 Resolved data from the XRPD pattern of the crystal form B No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) 1 5.462 16.1654 8.5 13 21.000 4.2268 100.0 2 10.806 8.1808 11.5 14 21.695 4.0930 25.8 3 11.042 8.0061 15.4 15 24.336 3.6545 26.4 4 12.067 7.3282 28.5 16 24.632 3.6113 12.1 5 12.700 6.9642 14.6 17 26.742 3.3309 36.0 6 13.328 6.6378 16.2 18 27.589 3.2305 30.8 7 14.890 5.9447 28.5 19 28.104 3.1725 27.0 8 16.010 5.5311 17.9 20 28.931 3.0836 9.6 9 17.194 5.1529 15.4 21 29.639 3.0116 18.6 10 18.300 4.8440 14.3 22 34.213 2.6187 12.3 11 18.887 4.6946 8.8 23 35.560 2.5225 11.0 12 19.933 4.4507 15.6

The present invention further provides a crystal form C of the compound of Formula (II), having an XRPD pattern with characteristic diffraction peaks at 2θ of 23.21°±0.20°, 24.30°±0.20°, and 27.71°±0.20°.

In some embodiments of the present invention, the crystal form C has an XRPD pattern with characteristic diffraction peaks at 2θ of 5.16±0.20°, 10.25±0.20°, 13.76±0.20°, 15.42±0.20°, 23.21±0.20°, 24.30±0.20°, 26.43±0.20°, and 27.71±0.20°.

In some embodiments of the present invention, the crystal form C has an XRPD pattern with characteristic diffraction peaks at 2θ of 5.161°, 10.250°, 13.765°, 15.420°, 15.619°, 22.423°, 23.214°, 24.296°, 26.430°, 27.711°, 28.381°, 29.997°, and 31.376°.

In some embodiments of the present invention, the crystal form C has an XRPD pattern as shown in FIG. 5 .

In some embodiments of the present invention, the resolved data from the XRPD pattern of the crystal form C are shown in Table 3:

TABLE 3 Resolved data from the XRPD pattern of the crystal form C No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) 1 5.161 17.1094 42.4 8 24.296 3.6604 55.6 2 10.250 8.6231 18.4 9 26.430 3.3695 19.2 3 13.765 6.4280 26.8 10 27.711 3.2165 56.2 4 15.420 5.7417 42.4 11 28.381 3.1421 18.6 5 15.619 5.6689 43.2 12 29.997 2.9764 16.9 6 22.423 3.9617 18.1 13 31.376 2.8487 15.5 7 23.214 3.8286 100.0

The present invention further provides a crystal form D of the compound of Formula (II), having an XRPD pattern with characteristic diffraction peaks at 2θ of 12.17°±0.20°, 26.12°±0.20°, and 28.59°±0.20°.

In some embodiments of the present invention, the crystal form D has an XRPD pattern with characteristic diffraction peaks at 2θ of 6.05±0.20°, 12.17±0.20°, 13.35±0.20°, 19.65±0.20°, 26.12±0.20°, 27.50±0.20°, 28.59±0.20°, and 30.08±0.20°.

In some embodiments of the present invention, the crystal form D has an XRPD pattern with characteristic diffraction peaks at 2θ of 6.05°, 10.86°, 12.17°, 13.35°, 13.76°, 14.90°, 15.70°, 16.90°, 17.70°, 18.30°, 18.91°, 19.65°, 20.14°, 20.68°, 21.37°, 21.67°, 22.08°, 23.17°, 23.67°, 23.90°, 25.25°, 25.80°, 26.12°, 26.70°, 26.90°, 27.50°, 27.79°, 28.59°, 29.02°, 30.08°, 30.51°, 30.78°, 30.87°, 31.89°, 32.28°, 32.49°, 33.30°, 34.77°, 35.44°, 35.85°, 36.49°, 37.36°, 37.93°, and 38.96°.

In some embodiments of the present invention, the crystal form D has an XRPD pattern as shown in FIG. 6 .

In some embodiments of the present invention, the resolved data from the XRPD pattern of the crystal form D are shown in Table 4:

TABLE 4 Resolved data from the XRPD pattern of the crystal form D No. 2θ (°) Interplanar spacing (Å) Intensity (counts) Relative intensity (%) No. 2θ (°) Interplanar spacing (Å) Intensity (counts) Relative intensity (%) 1 6.05 14.60 1493.65 47.99 23 26.12 3.41 3112.45 100.00 2 10.86 8.15 588.33 18.90 24 26.70 3.34 985.01 31.65 3 12.17 7.27 1586.82 50.98 25 26.90 3.31 1201.29 38.60 4 13.35 6.63 1495.75 48.06 26 27.50 3.24 1651.04 53.05 5 13.76 6.44 227.57 7.31 27 27.79 3.21 829.82 26.66 6 14.90 5.94 772.49 24.82 28 28.59 3.12 1847.45 59.36 7 15.70 5.64 452.25 14.53 29 29.02 3.08 325.63 10.46 8 16.90 5.25 519.41 16.69 30 30.08 2.97 141254 45.38 9 17.70 5.01 176.68 5.68 31 30.51 2.93 417.17 13.40 10 18.30 4.85 202.40 6.50 32 30.78 2.90 906.28 29.12 11 18.91 4.69 230.04 7.39 33 30.87 2.90 1070.89 34.41 12 19.65 4.52 1199.00 38.52 34 31.89 2.81 291.97 9.38 13 20.14 4.41 247.92 7.97 35 32.28 277 602.81 19.37 14 20.68 4.30 192.33 6.18 36 32.49 2.76 421.98 13.56 15 21.37 4.16 433.82 13.94 37 33.30 2.69 370.55 11.91 16 21.67 4.10 349.50 11.23 38 34.77 2.58 618.82 19.88 17 22.08 4.03 745.99 23.97 39 35.44 2.53 168.05 5.40 18 23.17 3.84 975.67 31.35 40 35.85 2.50 222.73 7.16 19 23.67 3.76 594.30 19.09 41 36.49 2.46 134.82 4.33 20 23.90 3.72 379.58 12.20 42 37.36 2.41 137.95 4.43 21 25.25 3.53 356.43 11.45 43 37.93 2.37 174.27 5.60 22 25.80 3.45 985.66 31.67 44 38.96 2.31 157.68 5.07

The present invention further provides a crystal form E of the compound of Formula (II), having an XRPD pattern with characteristic diffraction peaks at 2θ of 13.10°±0.20°, 14.50°±0.20°, and 24.87°±0.20°.

In some embodiments of the present invention, the crystal form E has an XRPD pattern with characteristic diffraction peaks at 2θ of 10.64±0.20°, 13.10±0.20°, 14.50±0.20°, 15.77±0.20°, 17.47±0.20°, 21.57±0.20°, 24.87±0.20°, and 27.42±0.20°.

In some embodiments of the present invention, the crystal form E has an XRPD pattern with characteristic diffraction peaks at 2θ of 10.64°, 13.10°, 14.50°, 15.77°, 17.47°, 20.17°, 21.57°, 22.28°, 23.87°, 24.87°, 26.02°, 27.42°, 28.67°, 30.18°, and 31.52°.

In some embodiments of the present invention, the crystal form E has an XRPD pattern as shown in FIG. 7 .

In some embodiments of the present invention, the resolved data from the XRPD pattern of the crystal form E are shown in Table 5:

TABLE 5 Resolved data from the XRPD pattern of the crystal form E No. 2θ (°) Interplanar spacing (Å) Intensity (counts) Relative intensity (%) No. 2θ (°) Interplanar spacing (Å) Intensity (counts) Relative intensity (%) 1 10.64 8.31 165.99 34.62 9 23.87 3.73 99.05 20.66 2 13.10 6.76 479.52 100.00 10 24.87 3.58 277.14 57.80 3 14.50 6.11 389.62 81.25 11 26.02 3.42 96.88 20.20 4 15.77 5.62 124.41 25.94 12 27.42 3.25 159.52 33.27 5 17.47 5.08 164.15 34.23 13 28.67 3.11 75.69 15.79 6 20.17 4.40 116.33 24.26 14 30.18 2.96 62.87 13.11 7 21.57 4.12 202.48 42.23 15 31.52 2.84 63.01 13.14 8 22.28 3.99 124.02 25.86

The present invention further provides a crystal form F of the compound of Formula (II), having an XRPD pattern with characteristic diffraction peaks at 2θ of 5.44°±0.20°, 14.06°±0.20°, and 14.92°±0.20°.

In some embodiments of the present invention, the crystal form F has an XRPD pattern with characteristic diffraction peaks at 2θ of 5.44±0.20°, 5.95±0.20°, 10.80±0.20°, 13.50±0.20°, 14.06±0.20°, 14.92±0.20°, 19.38±0.20°, and 27.57±0.20°.

In some embodiments of the present invention, the crystal form F has an XRPD pattern with characteristic diffraction peaks at 2θ of 5.44°, 5.95°, 8.94°, 9.34°, 10.80°, 11.25°, 11.91°, 13.50°, 14.06°, 14.92°, 16.60°, 19.38°, 23.97°, 24.90°, 26.21°, and 27.57°.

In some embodiments of the present invention, the crystal form F has an XRPD pattern as shown in FIG. 8 .

In some embodiments of the present invention, the resolved data from the XRPD pattern of the crystal form F are shown in Table 6:

TABLE 6 Resolved data from the XRPD pattern of the crystal form F No. 2θ (°) Interplanar spacing (Å) Intensity (counts) Relative intensity (%) No. 2θ (°) Interplanar spacing (Å) Intensity (counts) Relative intensity (%) 1 5.44 16.26 675.57 97.93 9 14.06 6.30 689.84 100.00 2 5.95 14.87 354.67 51.41 10 14.92 5.94 612.42 88.78 3 8.94 9.90 141.47 20.51 11 16.60 5.34 73.65 10.68 4 9.34 9.47 177.07 25.67 12 19.38 4.58 369.80 53.61 5 10.80 8.19 299.64 43.44 13 23.97 3.71 129.94 18.84 6 11.25 7.87 46.93 6.80 14 24.90 3.58 111.85 16.21 7 11.91 7.43 127.00 18.41 15 26.21 3.40 93.51 13.56 8 13.50 6.56 198.78 28.82 16 27.57 3.24 207.39 30.06

The present invention provides a compound of Formula (III):

The present invention further provides a crystal form G of the compound of Formula (III), having an XRPD pattern with characteristic diffraction peaks at 2θ of 6.86°±0.20°, 7.53°±0.20°, and 15.46°±0.20°.

In some embodiments of the present invention, the crystal form G has an XRPD pattern with characteristic diffraction peaks at 2θ of 6.86±0.20°, 7.53±0.20°, 9.21±0.20°, 9.80±0.20°, 10.70±0.20°, 13.06±0.20°, 15.46±0.20°, and 20.53±0.20°.

In some embodiments of the present invention, the crystal form G has an XRPD pattern with characteristic diffraction peaks at 2θ of 6.859°, 7.532°, 9.211°, 9.799°, 10.704°, 13.057°, 13.525°, 14.847°, 15.029°, 15.461°, 17.473°, 18.656°, 19.382°, 19.585°, 20.235°, 20.528°, 20.805°, 21.158°, 21.420°, 22.109°, 22.604°, 23.368°, 23.663°, 24.058°, 24.356°, 25.203°, 26.822°, 27.157°, 27.571°, 28.601°, 28.970°, 29.583°, 30.223°, 32.483°, 34.552°, 34.748°, and 35.268°.

In some embodiments of the present invention, the crystal form G has an XRPD pattern as shown in FIG. 9 .

In some embodiments of the present invention, the resolved data from the XRPD pattern of the crystal form G are shown in Table 7:

TABLE 7 Resolved data from the XRPD pattern of the crystal form G No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) 1 6.859 12.8757 57.2 20 22.109 4.0173 20.4 2 7.532 11.7279 100.0 21 22.604 3.9304 24.7 3 9.211 9.5936 30.1 22 23.368 3.8036 20.5 4 9.799 9.0187 49.3 23 23.663 3.7568 7.9 5 10.704 8.2584 50.2 24 24.058 3.6961 9.0 6 13.057 67747 30.5 25 24.356 3.6516 9.5 7 13.525 6.5412 8.9 26 25.203 3.5306 8.3 8 14.847 5.9617 5.8 27 26.822 3.3211 10.7 9 15.029 5.8899 7.7 28 27.157 3.2809 11.2 10 15.461 5.7265 61.5 29 27.571 3.2326 12.6 11 17.473 5.0712 5.7 30 28.601 3.1184 12.2 12 18.656 5.7524 9.0 31 28.970 3.0795 4.1 13 19.382 4.5758 19.8 32 29.583 3.0171 9.5 14 19.585 4.5290 17.2 33 30.223 2.9547 3.0 15 20.235 4.3849 6.2 34 32.483 27541 9.1 16 20.528 4.3229 25.3 35 34.552 2.5937 4.6 17 20.805 4.2659 12.0 36 34.748 2.5796 3.6 18 21.158 4.1957 5.3 37 35.268 2.5427 3.1 19 21.420 4.1449 9.4

In some embodiments of the present invention, the crystal form G has a differential scanning calorimetry (DSC) profile with an endothermic peak starting at 47.3±3.0° C., 86.8±3.0° C., and 145.2±3.0° C. respectively.

In some embodiments of the present invention, the crystal form G has a DSC profile as shown in FIG. 10 .

In some embodiments of the present invention, the crystal form G has a thermogravimetric analysis (TGA) curve showing a weight loss of 3.30% at 120.0° C.±3.0° C.

In some embodiments of the present invention, the crystal form G has a TGA curve as shown in FIG. 11 .

The present invention further provides a crystal form H of the compound of Formula (III), having an XRPD pattern with characteristic diffraction peaks at 2θ of 7.49°±0.20°, 15.24°±0.20°, and 22.03°±0.20°.

In some embodiments of the present invention, the crystal form H has an XRPD pattern with characteristic diffraction peaks at 2θ of 6.76±0.20°, 7.49±0.20°, 9.16±0.20°, 10.73±0.20°, 13.09±0.20°, 15.24±0.20°, 20.17±0.20°, and 22.03±0.20°.

In some embodiments of the present invention, the crystal form H has an XRPD pattern with characteristic diffraction peaks at 2θ of 6.76±0.20°, 7.49±0.20°, 9.18±0.20°, 10.73±0.20°, 13.09±0.20°, 15.24±0.20°, 20.17±0.20°, and 22.03±0.20°.

In some embodiments of the present invention, the crystal form H has an XRPD pattern with characteristic diffraction peaks at 2θ of 6.761°, 7.493°, 9.165°, 9.917°, 10.728°, 12.701°, 13.092°, 13.405°, 15.243°, 18.264°, 19.600°, 20.173°, 20.489°, 22.030°, 24.280°, 27.037°, 29.843°, and 35.438°.

In some embodiments of the present invention, the crystal form H has an XRPD pattern as shown in FIG. 12 .

In some embodiments of the present invention, the resolved data from the XRPD pattern of the crystal form H are shown in Table 8:

TABLE 8 Resolved data from the XRPD pattern of the crystal form H No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) 1 6.761 13.0631 45.8 10 18.264 4.8535 16.0 2 7.493 11.7883 87.0 11 19.600 4.5254 30.6 3 9.165 9.6411 29.9 12 20.173 4.3982 39.1 4 9.917 8.9115 46.3 13 20.489 4.3311 25.4 5 10.728 8.2395 35.7 14 22.030 4.0315 67.6 6 12.701 6.9642 15.1 15 24.280 3.6627 17.1 7 13.092 6.7569 47.9 16 27.037 3.2952 15.3 8 13.405 6.5997 24.0 17 29.843 2.9914 17.8 9 15.243 5.8077 100.0 18 35.438 2.5309 16.2

The present invention further provides a crystal form I of the compound of Formula (III), having an XRPD pattern with characteristic diffraction peaks at 2θ of 6.85°±0.20°, 7.49°±0.20°, and 15.42°±0.20°.

In some embodiments of the present invention, the crystal form I has an XRPD pattern with characteristic diffraction peaks at 2θ of 6.85±0.20°, 7.49±0.20°, 9.80±0.20°, 10.69±0.20°, 13.06±0.20°, 15.42±0.20°, 20.53±0.20°, and 22.64±0.20°.

In some embodiments of the present invention, the crystal form I has an XRPD pattern with characteristic diffraction peaks at 2θ of 6.854°, 7.494°, 9.171°, 9.797°, 10.688°, 13.055°, 14.810°, 15.422°, 18.676°, 19.383°, 19.620°, 20.527°, 20.881°, 22.105°, 22.640°, 23.389°, 24.094°, 24.356°, 25.185°, 26.802°, 27.432°, 28.633°, 29.561°, and 32.466°.

In some embodiments of the present invention, the crystal form I has an XRPD pattern as shown in FIG. 13 .

In some embodiments of the present invention, the resolved data from the XRPD pattern of the crystal form I are shown in Table 9:

TABLE 9 Resolved data from the XRPD pattern of the crystal form I No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) 1 6.854 12.88857 58.4 13 20.881 4.2507 19.0 2 7.494 11.7863 100.0 14 22.105 4.0181 28.2 3 9.171 9.6346 31.8 15 22.640 3.9243 40.8 4 9.797 9.0202 56.5 16 23.389 3.8003 29.9 5 10.688 8.2709 51.4 17 24.094 3.6906 9.5 6 13.055 6.7758 40.9 18 24.356 3.6515 13.8 7 14.810 5.9766 9.4 19 25.185 3.5332 13.1 8 15.422 57409 69.9 20 26.802 3.3236 16.9 9 18.676 4.7473 17.8 21 27.432 3.2486 20.5 10 19.383 4.5757 24.3 22 28.633 3.1151 18.4 11 19.620 4.5209 24.3 23 29.561 3.0193 13.0 12 20.527 4.3232 36.4 24 32.466 2.7555 12.9

The present invention provides a compound of Formula (IV):

The present invention further provides a crystal form J of the compound of Formula (IV), having an XRPD pattern with characteristic diffraction peaks at 2θ of 4.59°±0.20°, 7.02°±0.20°, and 18.05°±0.20°.

In some embodiments of the present invention, the crystal form J has an XRPD pattern with characteristic diffraction peaks at 2θ of 4.59±0.20°, 7.02±0.20°, 10.13±0.20°, 14.06±0.20°, 18.05±0.20°, 19.82±0.20°, 22.56±0.20°, and 27.04±0.20°.

In some embodiments of the present invention, the crystal form J has an XRPD pattern with characteristic diffraction peaks at 2θ of 4.592°, 6.094°, 7.018°, 9.383°, 10.132°, 11.535°, 12.241°, 14.059°, 18.046°, 19.819°, 21.435°, 22.561°, 23.764°, 24.117°, 26.489°, 27.035°, 28.732°, and 36.524°.

In some embodiments of the present invention, the crystal form J has an XRPD pattern as shown in FIG. 14 .

In some embodiments of the present invention, the resolved data from the XRPD pattern of the crystal form J are shown in Table 10:

TABLE 10 Resolved data from the XRPD pattern of the crystal form J No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) 1 4.592 19.2263 100.0 10 19.819 4.4759 5.7 2 6.094 14.4902 2.1 11 21.435 4.1420 1.6 3 7.018 12.5850 23.8 12 22.561 3.9377 4.3 4 9.383 9.4181 1.4 13 23.764 3.7411 1.7 5 10.132 8.7228 2.8 14 24.117 3.6871 2.7 6 11.535 7.6653 2.5 15 26.489 3.3621 1.3 7 12.241 7.2247 1.3 16 27.035 3.2954 3.3 8 14.059 6.2942 10.1 17 28.732 3.1045 2.5 9 18.046 4.9115 14.0 18 36.524 2.4581 1.2

In some embodiments of the present invention, the crystal form J has a thermogravimetric analysis (TGA) curve showing a weight loss of 4.97% at 120.0° C.±3.0° C.

In some embodiments of the present invention, the crystal form J has a TGA curve as shown in FIG. 15 .

The present invention further provides a crystal form K of the compound of Formula (IV), having an XRPD pattern with characteristic diffraction peaks at 2θ of 4.57°±0.20°, 18.02°±0.20°, and 19.76°±0.20°.

In some embodiments of the present invention, the crystal form K has an XRPD pattern with characteristic diffraction peaks at 2θ of 4.57±0.20°, 6.98±0.20°, 12.68±0.20°, 13.98±0.20°, 18.02±0.20°, 19.76±0.20°, 22.56±0.20°, and 26.96±0.20°.

In some embodiments of the present invention, the crystal form K has an XRPD pattern with characteristic diffraction peaks at 2θ of 4.570°, 6.111°, 6.980°, 9.069°, 10.173°, 11.023°, 11.591°, 12.680°, 13.980°, 15.953°, 17.119°, 18.024°, 19.760°, 20.213°, 20.942°, 21.419°, 22.560°, 24.081°, 24.611°, 26.960°, 28.121°, 28.575°, 29.640°, 31.733°, and 36.329°.

In some embodiments of the present invention, the crystal form K has an XRPD pattern as shown in FIG. 16 .

In some embodiments of the present invention, the resolved data from the XRPD pattern of the crystal form K are shown in Table 11:

TABLE 11 Resolved data from the XRPD pattern of the crystal form K No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) 1 4.570 19.3192 100.0 14 20.213 4.3897 5.6 2 6.111 14.4499 3.0 15 20.942 4.2384 5.0 3 6.980 12.6539 15.2 16 21.419 4.1451 3.3 4 9.069 9.7426 4.5 17 22.560 3.9380 12.2 5 10.173 8.6879 2.8 18 24.081 3.6925 6.7 6 11.023 8.0197 3.7 19 24.611 3.6143 5.6 7 11.591 7.6279 4.6 20 26.960 3.3045 7.2 8 12.680 6.9756 7.3 21 28.121 3.1706 3.5 9 13.980 6.3293 8.3 22 28.575 3.1212 4.4 10 15.953 5.5510 3.0 23 29.640 3.0114 2.5 II 17.119 5.1754 5.4 24 31.733 2.8175 2.5 12 18.024 4.9174 41.5 25 36.329 2.4708 3.7 13 19.760 4.4892 19.8

The present invention further provides a crystal form L of the compound of Formula (IV), having an XRPD pattern with characteristic diffraction peaks at 2θ of 4.61°±0.20°, 7.02°±0.20°, and 18.28°±0.20°.

In some embodiments of the present invention, the crystal form L has an XRPD pattern with characteristic diffraction peaks at 2θ of 4.61±0.20°, 7.02±0.20°, 11.83±0.20°, 18.28±0.20°, 20.27±0.20°, 21.50±0.20°, 22.58±0.20°, and 26.96±0.20°.

In some embodiments of the present invention, the crystal form L has an XRPD pattern with characteristic diffraction peaks at 2θ of 4.611°, 5.006°, 7.018°, 9.129°, 11.829°, 12.246°, 14.016°, 18.279°, 18.733°, 20.272°, 20.904°, 21.495°, 22.578°, 24.554°, 25.517°, 26.959°, 28.990°, and 29.603°.

In some embodiments of the present invention, the crystal form L has an XRPD pattern as shown in FIG. 17 .

In some embodiments of the present invention, the resolved data from the XRPD pattern of the crystal form L are shown in Table 12:

TABLE 12 Resolved data from the XRPD pattern of the crystal form L No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) 1 4.611 19.1486 100.0 10 20.272 4.3770 21.3 2 5.006 17.6369 32.5 11 20.904 4.2460 10.2 3 7.018 12.5852 52.7 12 21.495 4.1306 49.7 4 9.129 9.6797 7.6 13 22.578 3.9348 29.2 5 11.829 7.4750 12.6 14 24.554 3.6225 16.9 6 12.246 7.2219 7.5 15 25.517 3.4879 8.7 7 14.016 6.3135 11.6 16 26.959 3.3046 23.5 8 18.279 4.8494 55.0 17 28.990 3.0775 12.9 9 18.733 4.7329 15.5 18 29.603 3.0151 13.3

The present invention further provides a crystal form M of the compound of Formula (I), having an XRPD pattern with characteristic diffraction peaks at 2θ of 5.77°±0.20°, 12.63°±0.20°, and 15.40°±0.20°.

In some embodiments of the present invention, the crystal form M has an XRPD pattern with characteristic diffraction peaks at 2θ of 5.77±0.20°, 11.59±0.20°, 12.63±0.20°, 15.40±0.20°, 16.42±0.20°, 20.77±0.20°, 22.62±0.20°, and 23.36±0.20°.

In some embodiments of the present invention, the crystal form M has an XRPD pattern with characteristic diffraction peaks at 2θ of 5.77°, 5.95°, 10.52°, 11.59°, 12.63°, 13.12°, 15.40°, 16.42°, 16.94°, 18.05°, 20.03°, 20.77°, 21.44°, 22.62°, 22.98°, 23.36°, 24.46°, 25.82°, 26.54°, 27.31°, 27.96°, 29.70°, 31.17°, 32.04°, 33.16°, and 35.45°.

In some embodiments of the present invention, the crystal form M has an XRPD pattern as shown in FIG. 18 .

In some embodiments of the present invention, the resolved data from the XRPD pattern of the crystal form M are shown in Table 13:

TABLE 13 Resolved data from the XRPD pattern of the crystal form M No. 2θ (°) Interplanar spacing (Å) Intensity (counts) Relative intensity (%) No. 2θ (°) Interplanar spacing (Å) Intensity (counts) Relative intensity (%) 1 5.77 15.32 3437.28 44.97 14 22.62 3.93 652.45 8.54 2 5.95 14.87 2932.55 38.37 15 22.98 3.87 505.62 6.61 3 10.52 8.41 367.14 4.80 16 23.36 3.81 924.34 12.09 4 11.59 7.64 1696.52 22.20 17 24.46 3.64 155.16 2.03 5 12.63 7.01 2283.38 29.87 18 25.82 3.45 353.20 4.62 6 13.12 6.75 183.61 2.40 19 26.54 3.36 618.93 8.10 7 15.40 5.75 7643.69 100.00 20 27.31 3.27 160.35 2.10 8 16.42 5.40 860.98 11.26 21 27.96 3.19 141.66 1.85 9 16.94 5.23 277.21 3.63 22 2970 3.01 337.28 4.41 10 18.05 4.92 494.53 6.47 23 31.17 2.87 159.04 2.08 11 20.03 4.43 356.48 4.66 24 32.04 2.79 179.48 2.35 12 20.77 4.28 783.70 10.25 25 33.16 2.70 163.08 2.13 13 21.44 4.14 359.06 4.70 26 35.45 2.53 46.53 0.61

In some embodiments of the present invention, the crystal form M has a differential scanning calorimetry (DSC) profile with an endothermic peak starting at 74.4±3.0° C. and another endothermic peak starting at 214.3±3.0° C.

In some embodiments of the present invention, the crystal form M has a DSC profile as shown in FIG. 19 .

In some embodiments of the present invention, the crystal form M has a thermogravimetric analysis (TGA) curve showing a weight loss of 7.54% at 120.0° C.±3.0° C.

In some embodiments of the present invention, the crystal form M has a TGA curve as shown in FIG. 20 .

The present invention further provides a crystal form N of the compound of Formula (I), having an XRPD pattern with characteristic diffraction peaks at 2θ of 12.64°±0.20°, 17.10°±0.20°, and 20.92°±0.20°.

In some embodiments of the present invention, the crystal form N has an XRPD pattern with characteristic diffraction peaks at 2θ of 11.02±0.20°, 12.64±0.20°, 17.10±0.20°, 18.22±0.20°, 20.92±0.20°, 21.73±0.20°, 24.63±0.20°, and 26.65±0.20°.

In some embodiments of the present invention, the crystal form N has an XRPD pattern with characteristic diffraction peaks at 2θ of 9.111°, 11.022°, 12.642°, 13.289°, 15.934°, 16.626°, 17.096°, 18.221°, 18.753°, 19.876°, 20.234°, 20.922°, 21.733°, 22.659°, 22.972°, 24.631°, 25.416°, 25.776°, 26.646°, 27.454°, 28.103°, 28.360°, 28.835°, 29.561°, 32.683°, 34.041°, 35.459°, 36.959°, and 37.886°.

In some embodiments of the present invention, the crystal form N has an XRPD pattern as shown in FIG. 21 .

In some embodiments of the present invention, the resolved data from the XRPD pattern of the crystal form N are shown in Table 14:

TABLE 14 Resolved data from the XRPD pattern of the crystal form N No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) 1 9.111 9.6984 13.1 16 24.631 3.6114 45.6 2 11.022 8.0205 32.5 17 25.416 3.5015 5.1 3 12.642 6.9963 57.4 18 25.776 3.4535 11.3 4 13.289 6.6571 26.4 19 26.646 3.3427 35.2 5 15.934 5.5576 28.4 20 27.454 3.2461 26.6 6 16.626 5.3277 11.4 21 28.103 3.1725 23.8 7 17.096 5.1824 45.6 22 28.360 3.1444 5.9 8 18.221 4.8648 33.8 23 28.835 3.0937 12.1 9 18.753 4.7278 5.9 24 29.561 3.0194 18.7 10 19.876 4.4632 25.8 25 32.683 2.7377 4.6 11 20.234 4.3852 14.6 26 34.041 2.6315 5.3 12 20.922 4.2423 100.0 27 35.459 2.5295 5.7 13 21.733 4.0859 36.5 28 36.959 2.4302 4.9 14 22.659 3.9210 16.4 29 37.886 2.3728 4.1 15 22.972 3.8683 12.0

The present invention further provides a crystal form O of the compound of Formula (I), having an XRPD pattern with characteristic diffraction peaks at 2θ of 6.70°±0.20°, 10.41°±0.20°, and 13.74 ±0.20°.

In some embodiments of the present invention, the crystal form O has an XRPD pattern with characteristic diffraction peaks at 2θ of 4.63±0.20°, 6.70±0.20°, 9.80±0.20°, 10.41±0.20°, 13.74±0.20°, 14.16±0.20°, 16.57±0.20°, and 18.97±0.20°.

In some embodiments of the present invention, the crystal form O has an XRPD pattern with characteristic diffraction peaks at 2θ of 4.633°, 5.227°, 6.701°, 8.278°, 9.800°, 10.407°, 12.267°, 13.743°, 14.156°, 14.432°, 15.519°, 16.566°, 18.970°, 20.395°, and 24.479°.

In some embodiments of the present invention, the crystal form O has an XRPD pattern as shown in FIG. 22 .

In some embodiments of the present invention, the resolved data from the XRPD pattern of the crystal form O are shown in Table 15:

TABLE 15 Resolved data from the XRPD pattern of the crystal form O No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) No. 2θ (°) Interplanar spacing (Å) Relative intensity (%) 1 4.633 19.0585 43.8 9 14.156 6.2511 31.5 2 5.227 16.8934 22.8 10 14.432 6.1322 31.5 3 6.701 13.1801 97.6 11 15.519 5.7050 14.8 4 8.278 10.6725 11.8 12 16.566 5.3467 68.5 5 9.800 9.0182 84.1 13 18.970 4.6742 38.2 6 10.407 8.4936 94.3 14 20.395 4.3509 27.5 7 12.267 7.2096 11.3 15 24.479 3.6335 15.3 8 13.743 6.4383 100.0

The present invention further provides use of the compounds above, and use of the crystal form A, crystal form B, crystal form C, crystal form D, crystal form E, crystal form F, crystal form G, crystal form H, crystal form I, crystal form J, crystal form K, crystal form L, crystal form M, crystal form N or crystal form O of the compounds above in the preparation of medicine for treating FGFR and c-Met related diseases.

In some embodiments of the present invention, the medicine is used for the treatment of solid tumors.

Technical Effect

Various crystal form types of compounds of the present invention are stable and less affected by light, heat and humidity, have good therapeutic effect in vivo, thus having broad prospects in the preparation of drugs. Compared with the control, surprisingly, the crystal form A of the compound of Formula (II) has significantly improved activity against FGFR1 and FGFR4 and retains excellent activity against c-Met. The compounds of the present invention are obtained based on the structural analysis of the c-Met and FGFR kinase proteins, and a highly active small molecular nucleus that inhibits both c-Met and FGFR is found. The compounds of the present invention are an inhibitor against dual targets of the c-Met and FGFR. The FGFR and c-Met targets are synergistic and complementary, and the FGFR mutation or c-Met mutation tend to have a signal compensation effect when the other is inhibited, thus making the tumor cells resistant to a single inhibitor. Such inhibitors against dual targets will potentially reduce tumor cell-dependent escape and greatly improve the effect of tumor treatment. Crystal form A of the compound of Formula (II) exhibits a moderately low clearance, a high volume of distribution, a moderate half-life, and a high level of drug exposure when administered intravenously. When administered orally, crystal form A of the compound of Formula (II) shows a rapid peak time, and a high level of oral exposure, where the exposure level was higher than that of the crystal form G of the compound of Formula (III), and the crystal form J of the compound of Formula (III). The crystal form A of the compound of Formula (II) exhibits an excellent tumor inhibitory effect in the tumor model SNU-16.

Definition

Unless otherwise specified, the following terms and phrases used herein are intended to have the following meanings. A particular phrase or term should not be considered uncertain or unclear unless specifically defined, but should be understood in its ordinary meaning. When a trade name appears herein, it is intended to refer to the corresponding product or active ingredient.

The intermediate compounds of the present invention can be prepared by a variety of synthesis methods well known to those skilled in the art, including the specific embodiments listed below, the embodiments formed by the combination of the listed synthesis methods with other chemical synthesis methods, and equivalent alternatives well-known to those skilled in the art. Preferred embodiments include, but are not limited to, the examples of the present invention.

The chemical reactions in specific embodiments of the present invention are carried out in solvents suitable for the chemical changes and dissolving required reagents and materials of the present invention. To obtain the compounds of the present invention, it is sometimes necessary for those skilled in the art to modify or select the synthetic steps or reaction schemes on the basis of the embodiments below.

The structure of the compound of the present invention can be determined by conventional methods well known to those skilled in the art, and if the present invention relates to the absolute configuration of the compound, the absolute configuration can be confirmed by conventional technical means in the art. For example, in single crystal X-ray diffraction (SXRD), the grown single crystal is collected for the diffraction intensity data by BrukerD8 venture diffractometer with a light source of CuKα radiation in a scanning mode of φ/ω scanning. After relevant data is collected, the crystal structure is further resolved by a direct method (Shelxs97), to determine the absolute configuration.

The present invention will be specifically described below through the specific embodiments, and these embodiments do not imply any limitation to the present invention.

All solvents used in the present invention are commercially available and used without further purification.

The solvents used in the present invention are commercially available. The following abbreviations are used in the present invention: EtOH represents ethanol; MeOH represents methanol; TFA represents trifluoroacetic acid; TsOH represents p-toluenesulfonic acid; mp represents melting point; THF represents tetrahydrofuran; EtOAc represents ethyl acetate; and Pd(dppf)Cl₂ represents [1,1′-Bis(diphenylphosphino)ferrocene]palladium dichloride.

The compounds are named according to the conventional nomenclature in the art or by the ChemDraw® software, and the commercial compounds are named according to the directory name of the supplier.

X-Ray Powder Diffractometry (XRPD) of the Present Invention

-   Instrument model: X-ray powder diffractometer model X′Pert³ from     PANalytical -   Test method: About 10 mg sample is used for XRPD detection.

Detailed XRPD parameters:

-   Radiation source: Cu, kα (Kα1=1.540598 Å, Kα2=1.544426 Å, Kα2/Kα1     intensity ratio: 0.5) -   Tube voltage: 45 kV, Tube current: 40 mA -   Divergence slit: fixed ⅛ deg -   First Soller slit: 0.04 rad, Second Soller slit: 0.04 rad -   Receiving slit: No, Anti-scatter slit: 7.5 mm -   Measurement time: 5 min -   Sweep angle range: 3-40 deg -   Step width: 0.0263 deg -   Step length: 46.665 sec -   Rotation speed of sample pan: 15 rpm

Differential Scanning Calorimetry (DSC) of the Present Invention

-   Instrument model: TA5500 Differential Scanning Calorimeter -   Test method: A sample (about 1-5 mg) is placed in a DSC aluminum pan     and tested at a flow rate of nitrogen of 50 mL/min, by heating the     sample from 25° C. (room temperature) to the decomposition of the     sample at a ramping rate of 10° C./min.

Thermal Gravimetric Analysis (TGA) of the Present Invention

-   Instrument model: TA2500 Thermal Gravimetric Analyzer -   Test method: A sample (about 1-5 mg) is placed in a TGA aluminum pan     and tested at a flow rate of nitrogen of 10 mL/min, by heating the     sample from room temperature to 350° C. at a ramping rate of 10°     C./min.

Dynamic Vapor Sorption (DVS) of the Present Invention

-   Instrument model: SMS DVS Intrinsic Dynamic Vapor Sorption Analyzer -   Test method: A sample (10-30 mg) is tested in a DVS sample pan.

Detailed DVS parameters:

-   Temperature: 25° C. -   Equilibration: dm/dt=0.002%/min (Minimum: 10 min, Maximum: 180 min) -   RH (%) test interval: 10 (0-90%), 5 (90-95%) -   RH (%) test interval range: 0-95-0

Hygroscopicity evaluations are classified as follows: Classification of hygroscopicity ΔW% Deliquescent Absorb enough water to form a liquid Highly hygroscopic ΔW%≥15% Hygroscopic 15%>ΔW%≥2% Slightly hygroscopic 2%>ΔW%≥0.2% No or almost no hygroscopic ΔW%<0.2% Note: AW% represents the hygroscopic weight gain of the test product at 25° C./80%RH.

High Performance Liquid Chromatography (HPLC) of the Present Invention

Compound content test and analysis method Chromatographic column Waters Symmetry C18 (250 mm*4.6 mm, 5 µm) Mobile phase A 0.1% difluoroacetic acid in water Mobile phase B Acetonitrile Diluent/needle wash Acetonitrile: water=1:1 Flow rate 1.0 mL/min Volume of injection 10 μL Sample pan temperature Not controlled Column temperature 40° C. Detection wavelength 230 nm Time (min) Mobile phase A (%) Mobile phase B (%) Gradient elution program 0 70 30 10 70 30 10.1 10 90 15 10 90 15.1 70 30 25 70 30 Test sample concentration 0.1 mg/ml Quantification method External standard method

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is an XRPD pattern of the crystal form A of the compound of Formula (II) obtained using Cu-Kαradiation.

FIG. 2 is a DSC profile of the crystal form A of the compound of Formula (II).

FIG. 3 is a TGA curve of the crystal form A of the compound of Formula (II).

FIG. 4 is an XRPD pattern of the crystal form B of the compound of Formula (II) obtained using Cu-Kαradiation.

FIG. 5 is an XRPD pattern of the crystal form C of the compound of Formula (II) obtained using Cu-Kαradiation.

FIG. 6 is an XRPD pattern of the crystal form D of the compound of Formula (II) obtained using Cu-Kαradiation.

FIG. 7 is an XRPD pattern of the crystal form E of the compound of Formula (II) obtained using Cu-Kαradiation.

FIG. 8 is an XRPD pattern of the crystal form F of the compound of Formula (II) obtained using Cu-Kαradiation.

FIG. 9 is an XRPD pattern of the crystal form G of a compound of Formula (III) obtained using Cu-Kαα radiation.

FIG. 10 is a DSC profile of the crystal form G of the compound of Formula (III).

FIG. 11 is a TGA curve of the crystal form G of the compound of Formula (III).

FIG. 12 is an XRPD pattern of the crystal form H of the compound of Formula (III) obtained using Cu-Kαradiation.

FIG. 13 is an XRPD pattern of the crystal form I of the compound of Formula (III) obtained using Cu-Kαradiation.

FIG. 14 is an XRPD pattern of the crystal form J of a compound of Formula (IV) obtained using Cu-Kαradiation.

FIG. 15 is a TGA curve of the crystal form J of the compound of Formula (IV).

FIG. 16 is an XRPD pattern of the crystal form K of the compound of Formula (IV) obtained using Cu-Kαradiation.

FIG. 17 is an XRPD pattern of the crystal form L of the compound of Formula (IV) obtained using Cu-Kαradiation.

FIG. 18 is an XRPD pattern of the crystal form M of a compound of Formula (I) obtained using Cu-Kαradiation.

FIG. 19 is a DSC profile of the crystal form M of the compound of Formula (I).

FIG. 20 is a TGA curve of the crystal form M of the compound of Formula (I).

FIG. 21 is an XRPD pattern of the crystal form N of the compound of Formula (I) obtained using Cu-Kαradiation.

FIG. 22 is an XRPD pattern of the crystal form O of the compound of Formula (I) obtained using Cu-Kαradiation.

FIG. 23 is a DVS curve of the crystal form A of the compound of Formula (II).

FIG. 24 is a DVS curve of the crystal form G of the compound of Formula (III).

FIG. 25 is a DVS curve of the crystal form J of the compound of Formula (II).

DETAILED DESCRIPTION

For better understanding of the present invention, the present invention will be further described in conjunction with specific embodiments; however, these specific embodiments are not intended to limit the disclosure of the present invention.

Example 1: Preparation of Crystal Form A of Compound of Formula (II)

Synthesis route:

Step 1: Synthesis of Compound I-A

At 0° C., 3,4-dihydropyran (500.00 g, 5.94 mol, 543.48 mL) and concentrated hydrochloric acid (1.02 g, 10.35 mmol, 1.00 mL) were added to bromoethanol (500 g, 4.00 mol, 284.09 mL), and stirred at 19° C. for 1 hr. After the reaction was completed, 100 g of sodium bicarbonate was added and stirred for 30 min. The insolubles were removed by filtration to obtain a crude product. The filtrate was distilled under reduced pressure of 0.09 MPa, and the fraction at 80° C. was collected to obtain Compound I-A.

¹H NMR (400 MHz, CDCl₃) δ 4.66-4.60 (m, 1 H), 4.00-3.76 (m, 3 H), 3.50-3.47 (m, 1 H), 1.83-1.50 (m,6 H).

Step 2: Synthesis of Compound I-B

Compound I-A (600.78 g, 2.87 mol, 435.35 mL) and 4-boronate-1H-pyrazole (280 g, 1.44 mol) were dissolved in N,N-dimethylformamide (1120 mL), and then potassium carbonate (397.12 g, 2.87 mol) was added, and stirred at 60° C. for 48 hrs. TLC showed that the starting materials were reacted completely. The insolubles were removed by filtration, the filter cake was washed with ethyl acetate, the filtrates were combined, and the solvent was spun off to obtain the crude product. The crude product was purified by column chromatography on silica gel (heptane: ethyl acetate=8:1) to obtain Compound 1-B.

¹H NMR (400 MHz, CDCl₃) δ 7.84-7.78 (m, 1 H), 4.52-4.51 (m, 1 H), 4.35-4.32 (m, 2 H), 4.05-4.04 (m, 1 H), 3.79-3.45 (m, 3 H), 1.79-1.47 (m, 6 H), 1.32 (s, 12 H).

Step 3: Synthesis of Compound I-D

Compound I-C (250 g, 1.50 mol) and 1-chloromethyl-4-fluoro-1,4-diazabicyclo[2.2.2]octane bis(tetrafluoroborate) (1.33 kg, 3.76 mol) were added to acetonitrile (3750 mL), and reacted at 20-30° C. for 48 hrs. 19 L of water was slowly added to the reaction solution, and the reaction solution was filtered. The filter cake was washed with water (50 mL*2) to obtain a crude product. The crude product was purified by column chromatography (PE/DCM/EA=3/1/0-3/1/0.2). The obtained product (470 g) was added to n-heptane (2 L), and stirred at 10-20° C. for 30 min. The reaction system was stirred until uniform. After filtration, the filter cake was washed with n-heptane (50 mL) to obtain Compound I-D.

¹H HNMR (400 MHz, CDCl₃) δ 10.34-10.18 (m, 1 H), 6.90-6.73 (m, 1 H), 3.84 (s, 6 H).

Step 4: Synthesis of Compound I-F

Compound I-D (460 g, 2.28 mol) and Compound I-E (407.58 g, 2.07 mol) were added to methanol (1.8 L), and the reaction was heated to 40° C. A solution of potassium hydroxide (232.12 g, 4.14 mol) in methanol was added dropwise to the reaction flask, and the reaction was stirred at 40° C. for 10 min. The reaction temperature was lowered to 10 to 20° C. when the reaction solution was clear, and the reaction was stirred at this temperature for 16 hrs. The reaction solution was filtered under reduced pressure. The filter cake was washed with methanol (50 mL), and spin-dried under reduced pressure at 40-50° C. to obtain Compound I-F.

-   LCMS (ESI) m/z: 398.6 [M+1]⁺, 400.6 [M+1]⁺; -   ¹H NMR (400 MHz, CDC1₃) δ 8.24 (s, 1 H), 8.00 (s, 1 H), 7.30 (s, 1     H), 6.94 (t, J=8.0 Hz, 1 H), 3.84 (s, 6 H).

Step 5: Synthesis of Compound I-G

2.90 L of acetonitrile was added to a 5 L reaction flask at 10-25° C. Compound I-F (290.00 g) was added to the reaction flask in one portion with stirring at 10° C.-25° C., and then triethylsilane (232 mL) and trifluoroacetic acid (161 mL) were sequentially added dropwise to the reaction flask at 10° C.-25° C. The reaction was heated to an internal temperature of 55° C.-60° C., and the reaction was continuously stirred for 4 hrs at this temperature range. The reaction solution was cooled to room temperature (10-25° C.) and left to stand overnight. The reaction was filtered under reduced pressure, and the filter cake was rinsed with acetonitrile (100 mL*2). The filter cake was concentrated at a temperature of 40-50° C. under reduced pressure at a vacuum level of ≤-0.08 MPa by using an oil pump until no fractions were dropped, to obtain Compound I-G.

-   LCMS (ESI) m/z: 382.6 [M+1]⁺, 384.6 [M+1]⁺; -   ¹H NMR (400 MHz, DMSO-d₆) δ 11.75 (s, 1 H), 8.25 (s, 1 H), 8.09 (s,     1 H), 7.25 (s, 1 H), 6.88 (t, J=8.0 Hz, 1 H),4.02(s,2H),3.84(s,6H).

Step 6: Synthesis of Compound I-H

2.6 L of tetrahydrofuran and 0.52 L of distilled water were added into a 5 L reaction flask at 10-25° C. The content in the reaction flask was stirred and purged three times with nitrogen. Then, under nitrogen atmosphere, potassium carbonate (105.51 g) was added into the reaction flask. Compound I-G (130.00 g), Compound I-B (100.91 g) and Pd(dppf)Cl₂ (5.64 g) were added to the reaction flask under nitrogen atmosphere at 10° C.-25° C. The reaction was heated to an internal temperature of 65° C.-70° C., and the reaction was continuously stirred for 8-10 hrs at this temperature range. The reaction solution was cooled to room temperature (10-25° C.). Trithiocyanuric acid trisodium salt (130.00 g) was added in one portion, and stirred at 10° C.-25° C. for 16-20 hrs. The reaction solution was filtered through 200-300 g of diatomaceous earth, and then the filter cake was rinsed with tetrahydrofuran (120 mL*2). Trithiocyanuric acid trisodium salt (130.00 g) was added to the filtrate in one portion, and stirred at 10° C.-25° C. for 16-20 hrs. The reaction solution was filtered through 200-300 g of diatomaceous earth, and then the filter cake was rinsed with tetrahydrofuran (120 mL*2). 1.2 L of purified water was added to the filtrate, and the reaction solution was extracted with ethyl acetate (3.6 L*2). The combined organic phases were washed with saturated sodium chloride (1.2 L*2), and dried over anhydrous sodium sulfate (500 g) for 1-2 hrs. After filtration, the filtrate was concentrated under reduced pressure until no fractions were dropped to obtain a primary crude product. The primary crude product was purified by column chromatography to obtain a crude product. The crude product was dissolved in 1.6 L of ethyl acetate, heated to 45° C.-50° C., and cooled to 10° C.-25° C. after complete dissolution. 4.8 L of n-heptane was added dropwise over 0.5-1 hr with stirring. After a solid was precipitated, the reaction solution was continuously stirred at 10° C.-25° C. for 1 hr and filtered. The filter cake was washed with n-heptane (250 mL*2). The filter cake was concentrated under reduced pressure by using an oil pump until no fractions was dropped to obtain a crude product. The crude product was dissolved in 1.6 L of tetrahydrofuran at 10° C.-25° C., then about 250 g of a polymer resin PSB-22Y was added, heated to 55° C.-60° C., and stirred for 16-20 hrs at this temperature (300-305 r/mm). The liquid was filtered through 200-300 g of diatomaceous earth, and then rinsed with tetrahydrofuran (150 mL*2). Then about 250 g of PSB-22Y was added to the filtrate, heated to 55° C.-60° C., and stirred for 16-20 hrs (at a rotation speed of 300-305 r/mm). The liquid was filtered through 200-300 g of diatomaceous earth, and then rinsed with tetrahydrofuran (150 mL*2). The filtrate was concentrated under reduced pressure by a water pump until no fraction was dropped, wherein the temperature was 40° C.-50° C., and the vacuum level was <-0.08 MPa to obtain Compound I-H.

-   LCMS (ESI) m/z: 499.5 [M+1]⁺; -   ¹H NMR (400 MHz, DMSO-d₆) δ 11.75 (s, 1 H), 8.45 (s, 1 H), 8.15 (s,     1 H), 8.05 (s, 1 H), 7.87 (s, 1 H), 7.12 (s, 1 H), 6.88 (t, J=8.0     Hz, 1 H), 4.57 (s, 1 H), 4.38-4.32 (m, 2 H), 4.06 (s, 2 H),     3.97-3.92 (m, 1 H), 3.84 (s, 6 H), 3.79-3.78 (m, 1 H), 3.58-3.37(m,     2 H), 1.76-1.36 (m, 6 H).

Step 7: Synthesis of Crystal Form A of Compound of Formula (II)

2.78 L of ethanol was added to a 5 L three-neck flask at -25° C. After heating to 20-30° C., Compound I-H (139.00 g) was added with stirring, and the suspension was stirred at 20-30° C. for about 30 min. Hydrochloric acid/ethyl acetate (137 mL, 4 M) was added dropwise at 20-30° C. over 20 min. The reaction was mechanically stirred for 16-20 hrs at 20-30° C. Then the reaction solution was cooled to 10-25° C. and filtered under reduced pressure. The filter cake was rinsed with ethanol (200 mL*2). The filter cake was concentrated under reduced pressure until no fraction was dropped, wherein the temperature was 40-50° C., and the vacuum level was <-0.08 MPa to obtain the crystal form A of the compound of Formula (II).

-   LCMS (ESI) m/z: 415.0 [M+1]⁺; -   ¹H NMR (400 MHz, DMSO-d₆) δ 11.98 (s, 1 H), 8.58 (s, 1 H), 8.40 (s,     1 H), 8.24 (s, 1 H), 7.96 (s, 1 H), 7.19 (s, 1 H), 6.88 (t, J=8.0     Hz, 1 H), 4.57 (s, 1 H), 4.25-4.10 (m, 4 H), 3.85 (s, 6 H),     3.84-3.80 (m, 2 H).

Study of Salt Forming Number

From the molecular formula C₂₁H₂₀F₂N₄O₃HCl, the theoretical content of Cl ions was calculated to be 7.87%, which is very close to 7.04%. Therefore, the salt forming number of the compound of Formula (I) as a hydrochloride is 1.

Sample weight (mg) Test item Weight percent (w/w %) 1 41.7 Cl 7.1481 3 40.96 Cl 6.9307 Average Cl1 7.04

Example 2 Preparation of Crystal Form M of Compound of Formula (I)

50 mg of the crystal form A of the compound of Formula (II) was weighed, dissolved in water, dissociated with a saturated aqueous sodium bicarbonate solution, and stirred at 15° C. for 1 hr. The reaction solution was filtered, and the filter cake was subjected to rotary evaporation to dryness under reduced pressure, to obtain the crystal form M of the compound of Formula (I).

Preparation of Crystal Form A of Compound of Formula (II)

100 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, 4.0 mL of ethanol was added, then hydrogen chloride/ethyl acetate (4 M, 60 µL) was slowly added, and the reaction solution was stirred at 15° C. for 48 hrs. The reaction solution was filtered, and the filter cake was dried under reduced pressure (40° C.), to obtain the crystal form A of the compound of Formula (II).

350 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, 10 mL of methanol was added, and then 0.21 mL of hydrogen chloride/ethyl acetate (4 M) was slowly added to obtain a suspension. The suspension was stirred on a magnetic stirrer at 15° C. for 24 hrs (in the dark). The reaction solution was filtered, and the filter cake was dried in a vacuum drying oven (40° C.) to obtain the crystal form A of the compound of Formula (II).

Preparation of Crystal Form B of Compound of Formula (II)

100 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, 4.0 mL of acetone was added, then hydrogen chloride/ethyl acetate (4 M, 60 µL) was slowly added, and the reaction solution was stirred at 15° C. for 48 hrs. The reaction solution was filtered, and the filter cake was dried under reduced pressure (40° C.), to obtain the crystal form B of the compound of Formula (II).

Preparation of Crystal Form C of Compound of Formula (II)

50 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, 4.0 mL of ethyl acetate was added, then hydrogen chloride/ethyl acetate (4 M, 30 µL) was slowly added, and the reaction solution was stirred at 15° C. for 48 hrs. The reaction solution was filtered, and the filter cake was dried under reduced pressure (40° C.), to obtain the crystal form C of the compound of Formula (II).

Preparation of Crystal Form D of Compound of Formula (II)

350 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, 10 mL of acetone was added, and then 0.21 mL of hydrogen chloride/ethyl acetate (4 M) was slowly added to obtain a suspension. The suspension was stirred on a magnetic stirrer at 15° C. for 24 hrs (in the dark). The reaction solution was filtered, and the filter cake was dried in a vacuum drying oven (40° C.) to obtain the crystal form D of the compound of Formula (II).

350 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, 10 mL of ethyl acetate was added, and then 0.21 mL of hydrogen chloride/ethyl acetate (4 M) was slowly added to obtain a suspension. The suspension was stirred on a magnetic stirrer at 15° C. for 24 hrs (in the dark). The reaction solution was filtered, and the filter cake was dried in a vacuum drying oven (40° C.), to obtain the crystal form D of the compound of Formula (II).

350 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, 10 mL of tetrahydrofuran was added, and then 0.21 mL of hydrogen chloride/ethyl acetate (4 M) was slowly added to obtain a suspension. The suspension was stirred on a magnetic stirrer at 15° C. for 24 hrs (in the dark). The reaction solution was filtered, and the filter cake was dried in a vacuum drying oven (40° C.), to obtain the crystal form D of the compound of Formula (II).

350 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, 10 mL of acetone/water (v/v, 1/1) was added, and then 0.21 mL of hydrogen chloride/ethyl acetate (4 M) was slowly added to obtain a suspension. The suspension was stirred on a magnetic stirrer at 15° C. for 24 hrs (in the dark). The reaction solution was filtered, and the filter cake was dried in a vacuum drying oven (40° C.), to obtain the crystal form D of the compound of Formula (II).

Preparation of Crystal Form E of Compound of Formula (II)

350 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, 10 mL of tetrahydrofuran/water (v/v, 1/1) was added, and then 0.21 mL of hydrogen chloride/ethyl acetate (4 M) was slowly added to obtain a suspension. The suspension was stirred on a magnetic stirrer at 15° C. for 24 hrs (in the dark). The reaction solution was filtered, and the filter cake was dried in a vacuum drying oven (40° C.), to obtain the crystal form E of the compound of Formula (II).

Preparation of Crystal Form F of Compound of Formula (II)

350 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, 10 mL of ethanol/water (v/v, 1/1) was added, and then 0.21 mL of hydrogen chloride/ethyl acetate (4 M) was slowly added to obtain a suspension. The suspension was stirred on a magnetic stirrer at 15° C. for 24 hrs (in the dark). The reaction solution was filtered, and the filter cake was dried in a vacuum drying oven (40° C.), to obtain the crystal form F of the compound of Formula (II).

Preparation of Crystal Form G of Compound of Formula (III)

100 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, 4.0 mL of ethanol was added, then methanesulfonic acid (26 mg, 1.1 eq) was slowly added, and the reaction solution was stirred at 15° C. for 48 hrs. The reaction solution was filtered, and the filter cake was dried under reduced pressure (40° C.), to obtain the crystal form G of the compound of Formula (III).

¹H NMR (400 MHz, DMSO-d₆) δ 11.66 (s, 1 H), 8.51 (s, 1 H), 8.20 (s, 2 H), 7.90 (s, 1 H), 7.15 (s, 1 H), 6.90 (t, J=8.0 Hz, 1 H), 4.08-4.21 (m, 4 H), 3.84 (s, 6 H), 3.76-3.78 (m, 2 H), 2.33 (s, 3 H).

Preparation of Crystal Form H of Compound of Formula (III)

100 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, 4.0 mL of acetone was added, then methanesulfonic acid (26 mg, 1.1 eq) was slowly added, and the reaction solution was stirred at 15° C. for 48 hrs. The reaction solution was filtered, and the filter cake was dried under reduced pressure (40° C.), to obtain the crystal form H of the compound of Formula (III).

Preparation of Crystal Form I of Compound of Formula (III)

50 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, 4.0 mL of ethyl acetate was added, then methanesulfonic acid (13 mg, 1.1 eq) was slowly added, and the reaction solution was stirred at 15° C. for 48 hrs. The reaction solution was filtered, and the filter cake was dried under reduced pressure (40° C.), to obtain the crystal form I of the compound of Formula (III).

Preparation of Crystal Form J of Compound of Formula (IV)

100 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, 4.0 mL of ethanol was added, then p-toluenesulfonic acid (50 mg, 1.1 eq) was slowly added, and the reaction solution was stirred at 15° C. for 48 hrs. The reaction solution was filtered, and the filter cake was dried under reduced pressure (40° C.), to obtain the crystal form J of the compound of Formula (IV).

¹H NMR (400 MHz, DMSO-d₆) δ 11.91 (s, 1 H), 8.57 (s, 1 H), 8.40 (s, 1 H), 8.24 (s, 1 H), 7.95 (s, 1 H), 7.49-7.47 (m, 2 H), 7.21 (s, 1 H), 7.13-7.11 (m, 1 H), 6.90 (t, J=8.O Hz, 1 H), 4.22-4.11 (m, 4 H), 3.85 (s, 6 H), 3.81- 3.78 (m, 2 H), 2.29 (s, 3 H).

Preparation of Crystal Form K of Compound of Formula (IV)

100 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, 4.0 mL of acetone was added, then p-toluenesulfonic acid (50 mg, 1.1 eq.) was slowly added, and the reaction solution was stirred at 15° C. for 48 hrs. The reaction solution was filtered, and the filter cake was dried under reduced pressure (40° C.), to obtain the crystal form K of the compound of Formula (IV).

Preparation of Crystal Form L of Compound of Formula (IV)

50 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, 4.0 mL of ethyl acetate was added, then p-toluenesulfonic acid (25 mg, 1.1 eq.) was slowly added, and the reaction solution was stirred at 15° C. for 48 hrs. The reaction solution was filtered, and the filter cake was dried under reduced pressure (40° C.), to obtain the crystal form L of the compound of Formula (IV).

Preparation of Crystal Form N of Compound of Formula (I)

50 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, and 2.0 mL of acetone was added to obtain a suspension. The suspension was stirred on a magnetic stirrer (15° C.) for 16 hrs. The reaction solution was filtered, and the filter cake was dried under reduced pressure(40° C.), to obtain the crystal form N of the compound of Formula (I).

50 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, and 2.0 mL of tetrahydrofuran was added to obtain a suspension. The suspension was stirred on a magnetic stirrer (15° C.) for 16 hrs. The reaction solution was filtered, and the filter cake was dried under reduced pressure (40° C.), to obtain the crystal form N of the compound of Formula (I).

50 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, and 2.0 mL of ethanol was added to obtain a suspension. The suspension was stirred on a magnetic stirrer (15° C.) for 16 hrs. The reaction solution was filtered, and the filter cake was dried under reduced pressure(40° C.), to obtain the crystal form N of the compound of Formula (I).

50 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, and 2.0 mL of ethyl acetate was added to obtain a suspension. The suspension was stirred on a magnetic stirrer (15° C.) for 16 hrs. The reaction solution was filtered, and the filter cake was dried under reduced pressure(40° C.), to obtain the crystal form N of the compound of Formula (I).

50 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, and 2.0 mL of ethanol/water (v/v, 1/1) was added to obtain a suspension. The suspension was stirred on a magnetic stirrer (15° C.) for 16 hrs. The reaction solution was filtered, and the filter cake was dried under reduced pressure(40° C.), to obtain the crystal form N of the compound of Formula (I).

50 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, and 2.0 mL of acetone/water (v/v, 1/1) was added to obtain a suspension. The suspension was stirred on a magnetic stirrer (15° C.) for 16 hrs. The reaction solution was filtered, and the filter cake was dried under reduced pressure (40° C.), to obtain the crystal form N of the compound of Formula (I).

Preparation of Crystal Form O of Compound of Formula (I)

50 mg of the crystal form M of the compound of Formula (I) was weighed into a glass vial, and 2.0 mL of tetrahydrofuran/water (v/v, 1/1) was added to obtain a suspension. The suspension was stirred on a magnetic stirrer (15° C.) for 16 hrs. The reaction solution was filtered, and the filter cake was dried under reduced pressure (40° C.), to obtain the crystal form O of the compound of Formula (I).

Example 3 Hygroscopicity Test of Crystal Form A of Compound of Formula (II)

Experimental materials:

SMS DVS Intrinsic Dynamic Vapor Sorption Analyzer

Experimental method:

10-30 mg of the crystal form A of the compound of Formula (II) was placed in the DVS sample pan and tested.

Experimental results

The crystal form A of the compound of Formula (II) has a DVS curve as shown in FIG. 23 , wherein ΔW=0.3710%.

Experimental conclusions

The crystal form A of the compound of Formula (II) has a hygroscopic weight gain of 0.3710% at 25° C. and 80% RH, and is slightly hygroscopic.

Hygroscopicity Test of Crystal Form G of Compound of Formula (III)

Experimental materials:

SMS DVS Intrinsic Dynamic Vapor Sorption Analyzer

Experimental method:

10-30 mg of the crystal form G of the compound of Formula (III) was placed in the DVS sample pan and tested.

Experimental results

The crystal form G of the compound of Formula (III) has a DVS curve as shown in FIG. 24 , wherein ΔW=3.829%.

Experimental conclusions

The crystal form G of the compound of Formula (III) has a hygroscopic weight gain of 3.928% at 25° C. and 80% RH, and is hygroscopic.

Hygroscopicity Test of Crystal Form J of Compound of Formula (IV)

Experimental materials:

SMS DVS Intrinsic Dynamic Vapor Sorption Analyzer

Experimental method:

10-30 mg of the crystal form J of the compound of Formula (IV) was placed in the DVS sample pan and tested.

Experimental results

The crystal form J of the compound of Formula (IV) has a DVS curve as shown in FIG. 25 , wherein ΔW=6.077%.

Experimental conclusions

The crystal form J of the compound of Formula (IV) has a hygroscopic weight gain of 6.077% at 25° C. and 80% RH, and is hygroscopic.

To sum up: DVS data shows that hydrochloride is the least hygroscopic.

Example 4: Solubility Test of Crystal Form a of Compound of Formula (II) At Various pH

The solubility of the crystal form A of the compound of Formula (II) in 4 media with different pH values was tested. About 10 mg of the crystal form A of the compound of Formula (II) was weighed, and then 5.0 mL of different media (water, SGF, FaSSIF, and FeSSIF) were added respectively, to form a suspension. A magnetron was added to the suspension and the suspension was stirred on a magnetic stirrer for 2 hrs, and a sample was taken and centrifuged after 4 hrs. The sample in the upper layer was determined for concentration by HPLC and determined for the pH value. The test results are shown in Table 16:

TABLE 16 Solubility test results of crystal form A of compound of Formula (II) at various pH pH (24 h) State (24 h) Solubility (mg/ml)_24 h Water 2.42 Suspension 0.009 SGF 1.25 Suspension 0.112 FeSSIF 4.89 Suspension 0.252 FaSSIF 5.55 Suspension 0.004

-   •FaSSIF: (1). 0.042 g of sodium hydroxide, 0.3438 g of sodium     dihydrogen phosphate and 0.6186 g of sodium chloride were weighed,     added to 90 mL of purified water and mixed well. The solution was     adjusted to pH 6.5 with 1 N hydrochloric acid or 1 N sodium     hydroxide, and made up to 100 mL with purified water. (2). 0.224 g     of commercially available powder of FaSSIF/FeSSIF/FaSSGF was added     to 50 mL of the buffer, stirred until dissolved, and made up to 100     mL with purified water. The prepared buffer was left to stand at     room temperature for 2 hrs, and observed to be slightly milky white,     which was ready for use (simulating intestinal juice in human before     eating). -   •FeSSIF: (1). 0.404 g of sodium hydroxide, and 0.865 g of glacial     acetic acid, 1.1874 g of sodium chloride were weighed and added to     90 mL of purified water and mixed well. The solution was adjusted to     pH 5.0 with 1 N hydrochloric acid or 1 N sodium hydroxide, and made     up to 100 mL with purified water. (2). 1.12 g of commercially     available powder of FaSSIF/FeSSIF/FaSSGF was added to 50 mL of the     buffer, stirred until dissolved, and made up to 100 mL with purified     water. The prepared buffer was left to stand at room temperature for     2 hrs, and observed to be a clear liquid, which was ready for use     (simulating intestinal juice in human after eating). -   •FaSSGF (SGF): (1). 0.2 g of sodium chloride was weighed and added     to 90 mL of purified water and mixed well. The solution was adjusted     to pH 1.8 with 1 N hydrochloric acid, made up to 100 mL with     purified water, and left to stand until it was naturally cooled to     room temperature (simulating human gastric juice before eating).

Conclusions: The solubility data of the crystal form A of the compound of Formula (II) in biological media shows that it is hard to dissolve in water; the solubility in the simulated intestinal juice of the small intestine after eating in human and the simulated gastric juice before eating in human is good; and the solubility in the simulated intestinal juice before eating is poor.

Example 5: Solid Stability Test of Crystal Form A of Compound of Formula (II)

Experimental method: 12 parallel samples of the crystal form A of the compound of formula (II) were weighed in an amount of about 1 g each. Each sample was fed to a double-layer LDPE bag, and each layer of the LDPE bag was tied and sealed. Then the LDPE bag was placed in a bag of aluminum foil, and allowed to stand at 60° C./75%RH, 92.5%RH, and 40° C./75% RH to investigate the long-term accelerated stability. The photostability experiment was carried out as described in the Chinese Pharmacopoeia and ICH Q1B. The samples were irradiated with visible light and UV light.

The test results are shown in Table 17 below:

TABLE 17 Solid stability test results of crystal form A of compound of Formula (II) Test conditions Time point Appearance Crystal form (XRPD) Content (%) Total impurity (%) - Day 0 Off-white particles and powder Crystal form A 99.8 0.71 High temperature. (60° C., open) Day 5 Off-white particles and powder Crystal form A 99.6 0.78 Day 10 Off-white particles and powder Crystal form A 99.9 0.76 Day 30 Off-white particles and powder Crystal form A 98.5 0.80 High humidity (Room temperature/relative humidity 92.5%, open) Day 5 Off-white particles and powder Crystal form A 99.6 0.75 Day 10 Off-white particles and powder Crystal form A 99.8 0.71 Day 30 Off-white particles and powder Crystal form A 98.9 0.65 Light irradiation (General lighting: 1.2 × 10⁶Lux•hr/near ultraviolet: 200 w•hr/m², open 10 days) Light irradiation Off-white particles and powder Crystal form A 99.8 0.76 In the dark Off-white particles and powder Crystal form A 99.8 079 Acceleration experiment (40° C./relative humidty75%, open) 1 month Off-white particles and powder Crystal form A 99.7 0.67 2 month Off-white particles and powder Crystal form A 99.7 0.69 3 month Off-white particles and powder Crystal form A 99.9 0.68

Conclusions: With the accelerated conditions and affecting factors, the crystal form A of the compound of Formula (II) is very stable, and no obvious unknown impurities are formed.

Example 6: Stability Test of Crystal Form A and Crystal Form B of Compound of Formula (II)

35 g of the crystal form B of the compound of Formula (II) was weighed, added to ethanol, and stirred for 16 hrs. XRPD shows that the crystal form B of the compound of Formula (II) can be converted into the crystal form A of the compound of Formula (II). Therefore, the crystal form A of the compound of Formula (II) is more stable than the crystal form B of the compound of Formula (II).

Test Example 1: In-vitro Enzyme Activity Assay of the Compounds of the Present Invention

The IC₅₀ value was tested by ³³P isotope-labeled kinase activity assay (Reaction Biology Corp) to evaluate the inhibition of the test compounds on human FGFR1, FGFR4, and c-Met.

Buffer: 20 mM Hepes (pH 7.5), 10 mM MgC1₂, 1 mM EGTA, 0.02% Brij35, 0.02 mg/ml BSA, 0.1 mM Na₃VO₄, 2 mM DTT, and 1% DMSO.

Test procedure: At room temperature, the test compound was dissolved in DMSO to prepare a 10 mM solution for use. The substrate was dissolved in freshly prepared buffer, and the test kinase was added and mixed well. A solution of the test compound in DMSO was added to the mixed reaction solution using an acoustic technology (Echo550). The compound concentration in the reaction solution was 1 µM, 0.25 µM, 0.156 µM, 3.91 nM, 0.977 nM, 0.244 nM, 0.061 nM, 0.0153 nM, 0.00381 nM, or 10 µM, 2.50 µM, 0.62 µM, 0.156 µM, 39.1 nM, 9.8 nM, 2.4 nM, 0.61 nM, 0.15 nM, and 0.038 nM, respectively. After 15 min of incubation, ³³P-ATP (with an activity of 0.01 µCi/nL, the corresponding concentration is listed in Table 18) was added to start the reaction. The supplier’s catalogue number, lot number, and concentration in the reaction solution of FGFR1, FGFR4, c-Met, and substrates thereof are listed in Table 18. The reaction was carried out at room temperature for 120 min, and then the reaction solution was dotted on P81 ionexchange filter paper (Whatman #3698-915). The filter paper was repeatedly washed with 0.75% phosphoric acid solution, and then the radioactivity of the phosphorylated substrate remaining on the filter paper was determined. The kinase activity data were expressed by the comparison of the kinase activity in the presence of the test compound with the kinase activity of the blank group (containing only DMSO), and an IC₅₀ value was obtained by curve fitting with Prism4 software (GraphPad). The test results are shown in Table 19.

TABLE 18 Relevant information of kinases, substrates and ATP in in-vitro assays. Kinase Kinase concentration in the reaction solution (nM) Substrate Substrate concentration in the reaction solution (mg/ml) ATP concentration (µM) FGFR1 Supplier: Invitrogen Cat#: PV3146 Lot#: 28427Q 1.75 pEY(mg/ml)+Mn Supplier: Sigma Cat#: P7244-250MG Lot#: 062K5104V 0.2 mg/mL 5 FGFR4 Supplier: Invitrogen Cat#: P3054 Lot#: 26967J 2.5 pEY(mg/ml)+Mn Supplier: Sigma Cat#: P7244-250MG Lot#: 062K5104V 0.2 mg/mL 100 c-Met Supplier: Invitrogen Cat#: PV3143 Lot#: 464006A 8 MBP Supplier: Active Motif Cat#: 102641 Lot#: 04811001 20 µm 10

TABLE 19 IC₅₀ values of the kinase in the examples Test sample FGFR1 FGFR4 c-Met Comparative Example 1a 2419 >10000 758.3 Comparative Example 1b 709.1 5092.0 11.1 Crystal form A of compound of Formula (II) 0.20 1.49 15.9 Note: IC₅₀ is in nM.

Conclusions: Compared with the control, surprisingly, the crystal form A of the compound of Formula (II) has significantly improved activity against FGFR1 and FGFR4 and retains excellent activity against c-Met. The compounds of the present invention are obtained based on the structural analysis of the c-Met and FGFR kinase proteins, and a highly active small molecular nucleus that inhibits both c-Met and FGFR is found. The compounds of the present invention are an inhibitor against dual targets. The FGFR and c-Met targets are synergistic and complementary, and the FGFR mutation or c-Met mutation tend to have a signal compensation effect when the other is inhibited, thus making the tumor cells resistant to a single inhibitor. Such inhibitors against dual targets will potentially reduce tumor cell-dependent escape and greatly improve the effect of tumor treatment.

Test Example 2: Pharmacokinetic Evaluation of the Compounds of the Present Invention

Experiment procedure:

For agents for intravenous administration, 30% (v/v) PEG400 in purified water was as a vehicle. An appropriate amount of compound was accurately weighed into a glass flask, 30 vol% of PEG400 of the container volume was slowly added to the container with stirring, and then the remaining 70 vol% of pure water was added to make up to the final volume and stirred continuously until a clear solution was obtained. The solution was filtered through a 0.22 µM filter membrane, and stored at room temperature for later use. The agents for intravenous administration were formulated on the day of administration, and injected into male SD rats via tail vein (fasted overnight before administration) at a dose of 0.5 mg/kg. Approximately 0.18 mL of blood was collected from the jugular vein or tail vein at 0.0833, 0.25, 0.5, 1.0, 2.0, 4.0, 8.0 and 24 hrs after intravenous administration. The corresponding test compound in the vehicle was administered by gavage to male SD rats (fasted overnight before administration) at a dose of 5 mg/kg. The experimental conditions are detailed in Table 20. Blood was collected from the jugular or tail vein at 0.0833, 0.25, 0.5, 1.0, 2.0, 4.0, 6.0, 8.0 and 24 hrs after oral administration. The blood was fed to an anticoagulation tube supplemented with EDTA-K2, and centrifuged to separate plasma. The plasma concentrations were determined by LC-MS/MS, and the relevant pharmacokinetic parameters were calculated by the linear logarithmic trapezoid method of non-compartmental model using WinNonlm™ Version 6.3 (Pharsight, Mountain View, CA) pharmacokinetic software.

TABLE 20 Pharmacokinetic test conditions of compounds in rats IV (injection) PO (oral administration) Dose Vehicle Dose Vehicle Crystal form A of compound of Formula (II) 0.5 mg/kg 30% (v/v) PEG400 in purified water 5 mg/kg 1 mg/ mL in 35% PEG400 + 65% water Crystal form G of compound of Formula (III) / / 5 mg/kg 1 mg/mL in 35% PEG400 + 65% water Crystal form J of compound of Formula (IV) / / 5 mg/kg 1 mg/mL in 35% PEG400 + 65% water

TABLE 21 Pharmacokinetic test results of compounds in mice IV PO Dose 0.5 mg/kg 5 mg/kg Parameter Cl (mL/min/kg) Vdss (L/kg) T_(l/2) (h) AUC_(0-last) (nM•h) C_(max) (nM) T_(max) (h) AUC_(0-last) (nM•h) Crystal form A of compound of Formula (II) 18.4 1.87 1.32 1009 1960 0.5 5180 Crystal form G of compound of Formula (III) / / / / 903 0.75 3311 Crystal form J of compound of Formula (IV) / / / / 1083 0.5 3205 Note: “/” means not tested; plasma clearance (Cl), apparent volume of distribution at steady state (Vdss), elimination half-life (I_(½)), area under the plasma concentration curve from time point 0 to the last quantifiable time point (AUC_(0-last)), peak concentration (Cmax), and peak time (T_(max)).

Conclusions: when administered intravenously, crystal form A of the compound of Formula (II) exhibits moderately low clearance, high volume of distribution, moderate half-life, and high level of drug exposure. When administered orally, crystal form A of the compound of Formula (II) shows rapid peak time, and high level of oral exposure, where the exposure level was higher than that of the crystal form G of the compound of Formula (III), and the crystal form J of the compound of Formula (III).

Test Example 3: In Vivo Pharmacodynamic Evaluation of the Compounds of the Present Invention

SNU-16 cells in logarithmic growth phase were harvested, counted and resuspended in 50% PBS (pH 7.4, 0.01 M) and 50% Matrigel. The cell concentration was adjusted to 4 × 10⁷ cells/mL, and the cells were then placed in an ice box. The cell suspension was aspirated with a 1 mL syringe and injected subcutaneously into the anterior right axilla of nude mice, at a dose of 200 µL/animal (8 × 10⁶ cells/animal) to establish a SNU-16 xenograft model. The animal status was observed periodically, and the tumor size was measured using an electronic vernier caliper. The data was entered into an Excel spreadsheet, and the tumor volume was calculated, to monitor the tumor growth. When the tumor volume reached 100-300 mm³, 35 tumor-bearing mice (with a tumor volume of 112-182 mm³) in good health status having similar tumor volume were selected and randomized into 5 groups (n=7). The average tumor volume in each group was about 150 mm³. After the test was started, the tumor size was measured twice a week, the tumor volume was calculated, and the body weight of the animal was weighed and recorded.

Analysis of tumor growth inhibition (TGI): The evolutionary growth potential of tumors was evaluated by the relationship between the tumor volume and time. The long axis (L) and short axis (W) of subcutaneous tumor were measured twice a week by a caliper, and the tumor volume (TV) was calculated by the formula ((L×W²)/2). TGI was calculated from the difference between the median tumor volume of the mice in the solvent group and the median tumor volume of the mice in the drug group, and expressed as a percentage relative to the median tumor volume in the solvent control group.

TGI was calculated by the following formula:

$\begin{array}{l} {\%\text{TGI} = \left( \left( {\text{median tumor volume}\left( \text{control} \right)\text{-median tumor}} \right) \right)} \\ {\left( {\text{volume}\left( \text{administration group} \right)} \right)/\text{median tumor}} \\ {\left( {\text{volume}\left( \text{control group} \right)} \right) \times 100\%} \end{array}$

The test data was calculated and statistically processed by SPSS19.0. Unless otherwise specified, the data were expressed as mean ± standard error (Mean ± SE), and the comparison between the two groups was analyzed by T-test. P<0.05 indicates that there is a significant difference. The solvent 30% PEG400 (containing 70% deionized water, v/v) alone was used as the negative control. The experimental results are shown in Table 22.

TABLE 22 Test results of in-vivo antitumor activity in mice SNU-16 xenograft model TGI% (the last dose is given on day 30) p value Crystal form A of compound of Formula (II) 2.5 mg/kg BID 72 <0.005 Crystal form A of compound of Formula (II) 5.0 mg/kg BID 86 <0.005 Note: BID: twice a day, TGI%: tumor growth inhibition rate

Conclusions: The crystal form A of the compound of Formula (II) exhibited excellent tumor inhibitory effect in the tumor model SNU-16. 

What is claimed is:
 1. A crystal form A of a compound of Formula (II), having an X-ray powder diffraction pattern with characteristic diffraction peaks at 2θ of 5.40°±0.20°, 11.99°±0.20°, and 14.77°±0.20°

.
 2. The crystal form A according to claim 1, having an X-ray powder diffraction pattern with characteristic diffraction peaks at 2θ of 5.40±0.20°, 10.77±0.20°, 11.99±0.20°, 14.77±0.20°, 21.55±0.20°, 23.25±0.20°, 24.14±0.20°, and 27.69±0.20°.
 3. The crystal form A according to claim 2, having an X-ray powder diffraction pattern with characteristic diffraction peaks at 2θ of 5.402°, 8.949°, 10.766°, 11.989°, 13.186°, 14.766°, 16.090°, 16.779°, 19.721°, 21.554°, 23.251°, 23.685°, 24.138°, 25.224°, 27.690°, 28.670°, 29.287°, 31.378°, 33.941°, and 38.046°.
 4. (canceled)
 5. The crystal form A according to claim 1, having a differential scanning calorimetry profile with an endothermic peak starting at 220.0±3.0° C.
 6. (canceled)
 7. The crystal form A according to claim 1, having a thermogravimetric analysis curve showing a weight loss of 1.04% at 150.0° C.±3.0° C.
 8. (canceled)
 9. A compound of Formula (III):

.
 10. A crystal form G of the compound according to claim 9, having an X-ray powder diffraction pattern with characteristic diffraction peaks at 2θ of 6.86°±0.20°, 7.53°±0.20°, and 15.46°±0.20°.
 11. The crystal form G according to claim 10, having an X-ray powder diffraction pattern with characteristic diffraction peaks at 2θ of 6.86±0.20°, 7.53±0.20°, 9.21±0.20°, 9.80±0.20°, 10.70±0.20°, 13.06±0.20°, 15.46±0.20°, and 20.53±0.20°.
 12. The crystal form G according to claim 11, having an X-ray powder diffraction pattern with characteristic diffraction peaks at 2θ of 6.859°, 7.532°, 9.211°, 9.799°, 10.704°, 13.057°, 13.525°, 14.847°, 15.029°, 15.461°, 17.473°, 18.656°, 19.382°, 19.585°, 20.235°, 20.528°, 20.805°, 21.158°, 21.420°, 22.109°, 22.604°, 23.368°, 23.663°, 24.058°, 24.356°, 25.203°, 26.822°, 27.157°, 27.571°, 28.601°, 28.970°, 29.583°, 30.223°, 32.483°, 34.552°, 34.748°, and 35.268°.
 13. (canceled)
 14. The crystal form G according to claim 10, having a differential scanning calorimetry profile with an endothermic peak starting at 47.3±3.0° C., 86.8±3.0° C., and 145.2±3.0° C. respectively.
 15. (canceled)
 16. The crystal form G according to claim 10, having a thermogravimetric analysis curve showing a weight loss of 3.30% at 120.0° C.±3.0° C.
 17. (canceled)
 18. A compound of Formula (IV):

.
 19. A crystal form J of the compound according to claim 18, having an X-ray powder diffraction pattern with characteristic diffraction peaks at 2θ of 4.59°±0.20°, 7.02°±0.20°, and 18.05°±0.20°.
 20. The crystal form J according to claim 19, having an X-ray powder diffraction pattern with characteristic diffraction peaks at 2θ of 4.59±0.20°, 7.02±0.20°, 10.13±0.20°, 14.06±020°, 18.05±0.20°, 19.82±0.20°, 22.56±020°, and 27.04±0.20°.
 21. The crystal form J according to claim 20, having an X-ray powder diffraction pattern with characteristic diffraction peaks at 2θ of 4.592°, 6.094°, 7.018°, 9.383°, 10.132°, 11.535°, 12.241°, 14.059°, 18.046°, 19.819°, 21.435°, 22.561°, 23.764°, 24.117°, 26.489°, 27.035°, 28.732°, and 36.524°.
 22. (canceled)
 23. The crystal form J according to claim 19, having a thermogravimetric analysis curve showing a weight loss of 4.97% at 120.0° C.±3.0° C.
 24. (canceled)
 25. A crystal form N of a compound of Formula (I), having an X-ray powder diffraction pattern with characteristic diffraction peaks at 2θ of 12.64°±0.20°, 17.10°±0.20°, and 20.92°±0.20°.

.
 26. The crystal form N according to claim 25, having an X-ray powder diffraction pattern with characteristic diffraction peaks at 2θ of 11.02±0.20°, 12.64±0.20°, 17.10±0.20°, 18.22±0.20°, 20.92±0.20°, 21.73±0.20°, 24.63±0.20°, and 26.65±0.20°.
 27. The crystal form N according to claim 26, having an X-ray powder diffraction pattern with characteristic diffraction peaks at 2θ of 9.111°, 11.022°, 12.642°, 13.289°, 15.934°, 16.626°, 17.096°, 18.221°, 18.753°, 19.876°, 20.234°, 20.922°, 21.733°, 22.659°, 22.972°, 24.631°, 25.416°, 25.776°, 26.646°, 27.454°, 28.103°, 28.360°, 28.835°, 29.561°, 32.683°, 34.041°, 35.459°, 36.959°, and 37.886°.
 28. (canceled)
 29. Use of a compound of Formula (III) or a compound of Formula (IV), the crystal form A according to claim 1, or a crystal form N of a compound of Formula (I) in the preparation of medicine for treating FGFR and c-Met related diseases, wherein the crystal form N of the compound of Formula (I) has an X-ray powder diffraction pattern with characteristic diffraction peaks at 2θ of 12.64°±0.20°, 17.10°±0.20°, and 20.92°±0.20°;

.
 30. The use according to claim 29, wherein the medicine is a medicine for the treatment of solid tumors. 